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Question
Which of the following is used to determine the appropriate stringency (Wallace rule)?
Options:
A .  Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
B .  Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
C .  Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
D .  Tm = 69.3° + 0.41 (%)G+C -650/1
Answer: Option A
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