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Total Questions : 65 | Page 4 of 7 pages
Question 31. Which of the following conditions would cause the release of the lac repressor protein from the lac operator site on DNA?
  1.    Presence of IPTG (isopropyl thiogalactoside) in the growth media
  2.    Presence of glucose in the growth media
  3.    Presence of lactose in the growth media
  4.    Both (b) and (c)
 Discuss Question
Answer: Option D. -> Both (b) and (c)
Question 32.  The complex of RNA polymerase, DNA template and new RNA transcript is called
  1.    transcription bubble
  2.    replication bubble
  3.    a translation bubble
  4.    none of these
 Discuss Question
Answer: Option A. -> transcription bubble
Question 33.  The binding of lac repressor to DNA could be considered to be analogous to
  1.    competitive inhibition of an enzyme
  2.    mixed-type inhibition of an enzyme
  3.    uncompetitive inhibition of an enzyme
  4.    allosteric effects in enzyme regulation
 Discuss Question
Answer: Option A. -> competitive inhibition of an enzyme
Question 34.  Promoters for tRNAs are located
  1.    upstream from the start codon
  2.    downstream from the start codon
  3.    both (a) and (b)
  4.    none of these
 Discuss Question
Answer: Option B. -> downstream from the start codon
Question 35.  RNA polymerase in prokaryotes has a removable
  1.    alpha subunit
  2.    beta subunit
  3.    both (a) and (b)
  4.    sigma subunit
 Discuss Question
Answer: Option D. -> sigma subunit
Question 36.  Rho-dependent termination of transcription in E. coli
  1.    requires ATP
  2.    requires about 50 nucleotides of uncomplexed mRNA
  3.    both (a) and (b)
  4.    removes mRNA and holoenzyme from the DNA
 Discuss Question
Answer: Option C. -> both (a) and (b)
Question 37.  Enhancers are regions that
  1.    bind RNA polymerase
  2.    are adjacent to the TATA box
  3.    are CAT box binding proteins
  4.    modulate transcription
 Discuss Question
Answer: Option D. -> modulate transcription
Question 38.  Transcription by E. coli polymerase occurs in
  1.    four phases known as initiation, propagation, elongation and termination
  2.    three phases known as initiation, elongation and termination
  3.    two phases known as initiation and termination
  4.    none of the above
 Discuss Question
Answer: Option B. -> three phases known as initiation, elongation and termination
Question 39.  Which of the following is necessary for transcription to occur?
  1.    DNA molecule
  2.    RNA polymerase
  3.    DNA polymerase
  4.    Both DNA & RNA polymerase
 Discuss Question
Answer: Option D. -> Both DNA & RNA polymerase
Question 40. . In both eukaryotes and prokaryotes, a promoter region that is rich in AT bases is called as
  1.    CATT
  2.    Shine Dalgarno region
  3.    TATA box
  4.    SV40 region
 Discuss Question
Answer: Option C. -> TATA box

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