Cell Biology

TECHNIQUES IN CELL BIOLOGY MCQs

Total Questions : 116

Page 1 of 12 pages
Question 1. In mass-spectrometry, proteins are separated base on their _____________________
  1.    i-value
  2.    c-value
  3.    m/z ratio
  4.    e/m ratio
 Discuss Question
Answer is Option C. -> m/z ratio
Answer: (c).m/z ratio
Question 2. MALDI is a technique of ______________________
  1.    ionization
  2.    fractionation
  3.    proteolysis
  4.    cell counting
 Discuss Question
Answer is Option A. -> ionization
Answer: (a).ionization
Question 3. Electrospray ionization is suited for proteins that are __________________
  1.    in matrix
  2.    in glycerol
  3.    in solution
  4.    of large size
 Discuss Question
Answer is Option C. -> in solution
Answer: (c).in solution
Question 4. Which is the main ingredient in the sample preparation of mass spectrometry?
  1.    papain
  2.    pepsin
  3.    vinculin
  4.    trypsin
 Discuss Question
Answer is Option D. -> trypsin
Answer: (d).trypsin
Question 5. Which of the following techniques delivers the amino-acid sequence of a peptide?
  1.    Tandem MS
  2.    GC-MS
  3.    LC-MS
  4.    SDS-PAGE
 Discuss Question
Answer is Option A. -> Tandem MS
Answer: (a).Tandem MS
Question 6. The procedure by which DNA gel is transferred to a membrane is termed as ___________________
  1.    blotting
  2.    centifugation
  3.    electrophoresis
  4.    paper chromatography
 Discuss Question
Answer is Option A. -> blotting
Answer: (a).blotting
Question 7. In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography?
  1.    Western blotting
  2.    Southern blotting
  3.    Isopycnic centrifugation
  4.    Spectrophotometry
 Discuss Question
Answer is Option B. -> Southern blotting
Answer: (b).Southern blotting
Question 8. Which of the following molecules can be analyzed using a northern blot?
  1.    RNA
  2.    Carbohydrates
  3.    Proteins
  4.    DNA
 Discuss Question
Answer is Option A. -> RNA
Answer: (a).RNA
Question 9. Which of the following is a commonly used label in blotting techniques?
  1.    vimentin
  2.    biotin
  3.    avidin
  4.    streptomycin
 Discuss Question
Answer is Option B. -> biotin
Answer: (b).biotin
Question 10. Who developed the chemical techniques to synthesize polynucleotides?
  1.    Barbara McClintock
  2.    James Watson
  3.    Fredrick Sanger
  4.    H. Gobind Khorana
 Discuss Question
Answer is Option D. -> H. Gobind Khorana
Answer: (d).H. Gobind Khorana