Questions and answers for exam Preparation

MCQs

Total Questions : 71 | Page 1 of 8 pages

Question 1. For cloning, DNA samples are taken from

same individual
different individual
different species
none of above

Discuss Question

Answer: Option B. -> different individual
Answer: (b).different individual

Question 2. Choose the incorrect statement in respect to topoisomerase I.

It is used to cleave only one DNA strand
It leaves at the recognition sequence –C/TCCTT- and is covalently attached to one end of the cleaved molecule
The supercoiling of the DNA strand is altered and then it is sealed again by the enzyme
It is slower than the normal DNA ligase

Discuss Question

Answer: Option D. -> It is slower than the normal DNA ligase
Answer: (d).It is slower than the normal DNA ligase

Question 3. There is no effect if the insert itself contains the restriction site.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option B. -> False
Answer: (b).False

Question 4. Linkers are often used in cloning. Choose the incorrect statement for linkers.

These are short chemically synthesized molecules that contain a particular restriction enzyme site within the sequence
They are blunt ended molecules
They are ligated to staggered ended insert molecules by T4 DNA ligase
After treatment with enzyme, both the ends of the linker are staggered

Discuss Question

Answer: Option C. -> They are ligated to staggered ended insert molecules by T4 DNA ligase
Answer: (c).They are ligated to staggered ended insert molecules by T4 DNA ligase

Question 5. A small circular DNA present in bacterial cells are called as

enzymes
ribosomes
plasmids
Vector

Discuss Question

Answer: Option C. -> plasmids
Answer: (c).plasmids

Question 6. Generation of recombinants by randomly cloning fragments of total DNA from an organism is called as __________

genomic library
screening
recombination
shotgun cloning

Discuss Question

Answer: Option D. -> shotgun cloning
Answer: (d).shotgun cloning

Question 7. Insertion of DNA into lacZ gene may lead to disruption of the gene function.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 8. Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________

direct PCR
colony PCR
quantitative PCR
in-situ PCR

Discuss Question

Answer: Option B. -> colony PCR
Answer: (b).colony PCR

Question 9. Which of the following is true regarding taking up of plasmid DNA in the bacterial cells?

There are more chances of having two plasmids in a single cell
There are more chances of having a single plasmid in one cell
Uptake upto two plasmids is possible but not more than that
Both are taken up with the same efficiency

Discuss Question

Answer: Option B. -> There are more chances of having a single plasmid in one cell
Answer: (b).There are more chances of having a single plasmid in one cell

Question 10. If genomic DNA has been inserted instead of the plasmid, what will happen?