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MCQs

Total Questions : 282 | Page 5 of 29 pages
Question 41. Recombinant DNA molecule is produced by ligating __________
  1.    Two DNA segments
  2.    Two m-RNA molecules
  3.    One m-RNA with one t-RNA
  4.    One m-RNA with DNA segment
 Discuss Question
Answer: Option A. -> Two DNA segments
Answer: (a).Two DNA segments
Question 42. Where does a restriction endonuclease cuts DNA?
  1.    Ligation site
  2.    Recognition sequence
  3.    Ori
  4.    Replication site
 Discuss Question
Answer: Option B. -> Recognition sequence
Answer: (b).Recognition sequence
Question 43. Arrange the following steps involved in Gene cloning in the correct order.
i) Isolation of desired gene
ii) Insertion of isolated gene to the vector
iii) Introduction of recombinant vector to the host
iv) Expression of recombinant gene in host
v) Extraction of recombinant gene product
  1.    i, ii, iii, iv, v
  2.    v, iv, iii, ii, i
  3.    i, iii, ii, iv, v
  4.    iv, v, ii, i, iii
 Discuss Question
Answer: Option A. -> i, ii, iii, iv, v
Answer: (a).i, ii, iii, iv, v
Question 44. Which of the following statements is true about a vector?
  1.    Plasmids, phages can be used as vectors
  2.    All vectors are plasmids
  3.    Cyanobacteria can also be used as vectors
  4.    Fungi can also be used as vectors
 Discuss Question
Answer: Option A. -> Plasmids, phages can be used as vectors
Answer: (a).Plasmids, phages can be used as vectors
Question 45. Which of the following is true about plasmids?
  1.    They are present in all organisms
  2.    They are present in bacteria only
  3.    They are present in bacteria and phages
  4.    They are present in plants and animals
 Discuss Question
Answer: Option C. -> They are present in bacteria and phages
Answer: (c).They are present in bacteria and phages
Question 46. Which of the following codons is not a termination codon for protein synthesis?
  1.    UUU
  2.    UAG
  3.    UAA
  4.    UGA
 Discuss Question
Answer: Option A. -> UUU
Answer: (a).UUU
Question 47. A set of overlapping DNA segments that together represent a consensus region of DNA is ____________
  1.    Expressed sequence tag
  2.    Sequence tagged site
  3.    Contig
  4.    YAC
 Discuss Question
Answer: Option C. -> Contig
Answer: (c).Contig
Question 48. Short DNA sequence having single occurrence in genome is ____________
  1.    Expressed sequence tag
  2.    Sequence tagged site
  3.    Contig
  4.    YAC
 Discuss Question
Answer: Option B. -> Sequence tagged site
Answer: (b).Sequence tagged site
Question 49. Short sub-sequence of a cDNA sequence is ____________
  1.    Expressed sequence tag
  2.    Sequence tagged site
  3.    Contig
  4.    YAC
 Discuss Question
Answer: Option A. -> Expressed sequence tag
Answer: (a).Expressed sequence tag
Question 50. Which of the following are the two methods of screening?
  1.    Hybridization and PCR
  2.    ELISA and blotting
  3.    ELISA and PCR
  4.    PCR and RFLP
 Discuss Question
Answer: Option A. -> Hybridization and PCR
Answer: (a).Hybridization and PCR

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