Questions and answers for exam Preparation

MCQs

Total Questions : 72 | Page 1 of 8 pages

Question 1. By using two oligonucleotides, we can measure the amount of quantity of DNA by measuring the amount of fluorescence. The first probe absorbs light and the second emits it at a particular wavelength.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 2. Choose the correct statement with regard to quantitative PCR?

End-point PCR is favourable over real time PCR
In real time PCR, quantification is done as the reaction is going on
If the product measurement is done after the completion then the measurement is done by target sequence and no other factor affects it
If the primers are available in limited amount, then the product obtained is proportional to the target sequence

Discuss Question

Answer: Option B. -> In real time PCR, quantification is done as the reaction is going on
Answer: (b).In real time PCR, quantification is done as the reaction is going on

Question 3. If amplification of one of the strand is favoured, the modification of PCR is known as __________

single-strand PCR
partial PCR
asymmetric PCR
anchored PCR

Discuss Question

Answer: Option C. -> asymmetric PCR
Answer: (c).asymmetric PCR

Question 4. Which of the statement is incorrect for anchored PCR?

Anchored PCR is the modification in which only one piece of the sequence is known and thus one primer
The known sequence is attached to the required region of amplification and then further used as a second priming site
Fragment the sample DNA and ligate it to known sequence
Tails are added enzymatically to the region of known sequence

Discuss Question

Answer: Option D. -> Tails are added enzymatically to the region of known sequence
Answer: (d).Tails are added enzymatically to the region of known sequence

Question 5. Mutation is never deliberately induced in PCR products.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option B. -> False
Answer: (b).False

Question 6. Which of the following is not a condition for PCR?

Initial melting carried out for 5 minutes at 94 degrees
Initial melting followed by 30 cycles each consisting of melting for 1 minute at 94 degrees
Renaturation for 5 minutes at 60 degrees
DNA synthesis at 72 degrees for 1.5 minutes

Discuss Question

Answer: Option C. -> Renaturation for 5 minutes at 60 degrees
Answer: (c).Renaturation for 5 minutes at 60 degrees

Question 7. Primers and polymerases are added again during the reaction because they get consumed as the reaction proceeds.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option B. -> False
Answer: (b).False

Question 8. Which of the following activity is not present in Taq polymerase?

5’-3’ polymerase
5’-3’ exonuclease
3’-5’ exonuclease
Both 5’-3’ polymerase and 5’-3’ exonuclease

Discuss Question

Answer: Option C. -> 3’-5’ exonuclease
Answer: (c).3’-5’ exonuclease

Question 9. All the molecules generated during PCR will not be full length. Some will also be of intermediate length. Which of the statements is correct?

After first cycle, majority of the molecules will be full length and only some will be of intermediate length
In the next cycle, each intermediate molecule will generate one intermediate molecule and one target molecule
The number of full length molecules increase as number of cycles proceed
The number of intermediate molecules increase geometrically and the number of target molecules increase arithmetically

Discuss Question

Answer: Option B. -> In the next cycle, each intermediate molecule will generate one intermediate molecule and one target molecule
Answer: (b).In the next cycle, each intermediate molecule will generate one intermediate molecule and one target molecule

Question 10. Choose the correct statement.

Taq polymerase is having high processivity
Processivity is defined in this case as a synthesis of DNA by polymerase
It requires a 5’ end for the elongation to take place
The maximum size of the molecules which can be synthesized is 10kbp

Discuss Question

Answer: Option B. -> Processivity is defined in this case as a synthesis of DNA by polymerase
Answer: (b).Processivity is defined in this case as a synthesis of DNA by polymerase