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MCQs

Total Questions : 26 | Page 2 of 3 pages
Question 11. Transformation means
  1.    acquiring DNA from the bacterial cell environment
  2.    F+ and F- strains of bacteria
  3.    formation of a pilus
  4.    plasmid containing a F factor
 Discuss Question
Answer: Option A. -> acquiring DNA from the bacterial cell environment
Question 12. Restriction maps
  1.    does not allow comparison between DNA molecules
  2.    None of these
  3.    allows comparison between DNA molecules but requires to determine nucleotide sequence
  4.    allows comparison between DNA molecules without the need to determine nucleotide sequence
 Discuss Question
Answer: Option D. -> allows comparison between DNA molecules without the need to determine nucleotide sequence
Question 13. PCR can be used in
  1.    All of these
  2.    cloning
  3.    medical diagnosis and forensic medicine
  4.    sequencing
 Discuss Question
Answer: Option A. -> All of these
Question 14. A PCR cycle consists of
  1.    three steps, denaturation, initiation and elongation
  2.    three steps, initiation, elongation and termination
  3.    three steps, primer annealing, elongation and termination
  4.    three steps, denaturation, primer annealing and elongation
 Discuss Question
Answer: Option D. -> three steps, denaturation, primer annealing and elongation
Question 15. How many ds DNA molecule can be produced, which comprise precisely the target region in double strand form during 4th cycle of PCR?
  1.    Four ds DNA molecule
  2.    Two ds DNA molecule
  3.    Three ds DNA molecule
  4.    Eight ds DNA molecule
 Discuss Question
Answer: Option A. -> Four ds DNA molecule
Question 16. PCR (Polymerase Chain Reaction) can make multiple copies of
  1.    protein
  2.    carbohydrate
  3.    DNA
  4.    RNA
 Discuss Question
Answer: Option C. -> DNA
Question 17. Bactriophages absorb to a bacterial surface and inject the phage DNA through the
  1.    both (a) and (b)
  2.    cell wall into cytosol
  3.    cell wall into endoplasmic reticulum
  4.    cell wall into plasmamembrane
 Discuss Question
Answer: Option B. -> cell wall into cytosol
Question 18. PCR is an abbreviation for
  1.    Positive Charge Reaction:
  2.    Preliminary Change Rate
  3.    Polymerase Chain Reaction
  4.    Protein Change Reaction
 Discuss Question
Answer: Option C. -> Polymerase Chain Reaction
Question 19. Southern blotting involves
  1.    electrophoresis of RNA molecules and then blotting the separated RNA bands followed by incubation
  2.    electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation
  3.    electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation
  4.    electrophoresis of DNA molecules followed by incubation
 Discuss Question
Answer: Option B. -> electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation
Question 20. PCR amplifies millions of copies of
  1.    a short fragment of DNA
  2.    short chain RNA
  3.    both (a) and (b)
  4.    the entire genome (all the DNA in a sample)
 Discuss Question
Answer: Option A. -> a short fragment of DNA

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