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PCR MCQs

Total Questions : 21 | Page 2 of 3 pages
Question 11. How many cycles of PCR does it take to produce a double-stranded DNA fragment of the desired length?
  1.    1
  2.    2
  3.    3
  4.    4
 Discuss Question
Answer: Option C. -> 3
Question 12. What is the purpose of the Extension step of PCR?
  1.    To create the primers.
  2.    To extend the time it takes to produce DNA.
  3.    To allow polymerase to create the complementary strands of DNA.
  4.    To allow substrates to copy DNA sequences.
 Discuss Question
Answer: Option C. -> To allow polymerase to create the complementary strands of DNA.
Question 13. How many cycles of PCR are normally used
  1.    20-35
  2.    6
  3.    45
  4.    100
 Discuss Question
Answer: Option A. -> 20-35
Question 14. What happens during the Anneal step of PCR?
  1.    Primers are created.
  2.    The primers attach to the target DNA region.
  3.    Primers copy the new DNA strand.
  4.    Primers sequence DNA
 Discuss Question
Answer: Option B. -> The primers attach to the target DNA region.
Question 15. Starting with 15N15N (heavy) DNA, and after one generation in the 14N medium, Escherichia coli cells will contain
  1.    all 15N14N (hybrid) DNA.
  2.    half 15N15N DNA (heavy) and half 14N14N DNA (light).
  3.    half 14N14N (light) DNA and half 15N14N (hybrid) DNA.
  4.    all 14N14N (light) DNA.
 Discuss Question
Answer: Option A. -> all 15N14N (hybrid) DNA.
Question 16. Which of the following statements are true regarding PCR
  1.    Automated PCR machines are called thermal cyclers
  2.    A thermostable DNA Polymerase is required
  3.    Billion copies of desired DNA can be synthesized from microgram quantities of DNA
  4.    All of these
 Discuss Question
Answer: Option D. -> All of these
Question 17. The basic requirements of PCR reaction include
  1.    DNA segment to be amplified
  2.    Two oligonucleotide primers
  3.    a heat stable DNA polymerase
  4.    all of these
 Discuss Question
Answer: Option D. -> all of these
Question 18. You decide to use PCR to determine if you have Cystic Fibrosis, a genetic disorder
  1.    Template DNA
  2.    Nucleotides
  3.    Taq DNA polymerase
  4.    Primers
 Discuss Question
Answer: Option D. -> Primers
Question 19. Which of the following statements about DNA replication is TRUE?
  1.    the leading strand is replicated continuously, while the lagging strand is replicated discontinuously
  2.    the leading strand is replicated discontinuously, while the lagging strand is replicated continuously
  3.    both the leading and lagging strands are replicated continuously
  4.    both the leading and lagging strands are replicated discontinuously
 Discuss Question
Answer: Option A. -> the leading strand is replicated continuously, while the lagging strand is replicated discontinuously
Question 20. Thermus aquaticus is the source of
  1.    Primase enzyme
  2.    Taq Polymerase
  3.    Vent Polymerase
  4.    Both 2 and 3
 Discuss Question
Answer: Option D. -> Both 2 and 3

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