Pcr(General Knowledge > General Science ) Questions and answers for exam Preparation

PCR MCQs

Total Questions : 21 | Page 1 of 3 pages

Question 1. How is Fast PCR able to run at such a shorter time frame

Lower the temperature of Denaturation
raises the temperature for annealing so that it and extension can happen almost simultaneously
Shortens the time frame of denatureation
all of the answers are correct

Discuss Question

Answer: Option D. -> all of the answers are correct

Question 2. What is the name of the enzyme used to extend the target gene?

Discuss Question

Answer: Option B. -> Taq polymerase

Question 3. What is the best definition for "dNTP"

A nitrogenous base bound to a 5 carbon sugar with 3 phosphate groups bound to the sugar.
A nitrogenous base bound to a 5 carbon sugar.
The generic name for a nucleotide base.
The generic name for a nucleoside base.

Discuss Question

Answer: Option A. -> A nitrogenous base bound to a 5 carbon sugar with 3 phosphate groups bound to the sugar.

Question 4. Why must annealing of primers take place at between 50 - 65oC?

To prevent Taq polymerase from denaturing, allowing it to catalyse the binding of primers to the target gene.
To ensure that primer annealing happens quickly - higher rate of reaction as temperature is high.
To allow the formation of hydrogen bonds between the primer and target gene.
To prevent the formation of hydrogen bonds between the primer and target gene.

Discuss Question

Answer: Option C. -> To allow the formation of hydrogen bonds between the primer and target gene.

Question 5. What is the best definition for "primer"?

An oligonucleotide of unknown sequence that is used to amplify the target gene.
An oligonucleotide of known sequence that is used to amplify the target gene.
An oligonucleotide of known sequence that is annealed to the target gene, allowing Taq polymerase to bind.
A section of DNA on the target gene that is the binding site for Taq polymerase.

Discuss Question

Answer: Option C. -> An oligonucleotide of known sequence that is annealed to the target gene, allowing Taq polymerase to bind.

Question 6. What type of bonds hold the two strands of DNA together?

Discuss Question

Answer: Option A. -> Hydrogen bonds

Question 7. What is the function of DNA Polymerase?

Discuss Question

Answer: Option C. -> attach nucleotides to the target sequence

Question 8. Which of the following is true regarding PCR

Discuss Question

Answer: Option D. -> All of these

Question 9. Why does PCR require a specific type of polymerase?

Taq polymerase can withstand the range of temperatures in PCR without denaturing.
Taq polymerase is the most inexpensive type of polymerase.
Taq polymerase is the most common type of polymerase.
A specific type of polymerase is unnecessary.

Discuss Question

Answer: Option A. -> Taq polymerase can withstand the range of temperatures in PCR without denaturing.

Question 10. At what temperature does the Denature step of PCR occur?