General Science

PCR MCQs

Total Questions : 21

Page 1 of 3 pages
Question 1. How is Fast PCR able to run at such a shorter time frame
  1.    Lower the temperature of Denaturation
  2.    raises the temperature for annealing so that it and extension can happen almost simultaneously
  3.    Shortens the time frame of denatureation
  4.    all of the answers are correct
 Discuss Question
Answer is Option D. -> all of the answers are correct
Question 2. What is the name of the enzyme used to extend the target gene?
  1.    Tac polymerase
  2.    Taq polymerase
  3.    DNA polymerase
  4.    Yak polymerase
 Discuss Question
Answer is Option B. -> Taq polymerase
Question 3. What is the best definition for "dNTP"
  1.    A nitrogenous base bound to a 5 carbon sugar with 3 phosphate groups bound to the sugar.
  2.    A nitrogenous base bound to a 5 carbon sugar.
  3.    The generic name for a nucleotide base.
  4.    The generic name for a nucleoside base.
 Discuss Question
Answer is Option A. -> A nitrogenous base bound to a 5 carbon sugar with 3 phosphate groups bound to the sugar.
Question 4. Why must annealing of primers take place at between 50 - 65oC?
  1.    To prevent Taq polymerase from denaturing, allowing it to catalyse the binding of primers to the target gene.
  2.    To ensure that primer annealing happens quickly - higher rate of reaction as temperature is high.
  3.    To allow the formation of hydrogen bonds between the primer and target gene.
  4.    To prevent the formation of hydrogen bonds between the primer and target gene.
 Discuss Question
Answer is Option C. -> To allow the formation of hydrogen bonds between the primer and target gene.
Question 5. What is the best definition for "primer"?
  1.    An oligonucleotide of unknown sequence that is used to amplify the target gene.
  2.    An oligonucleotide of known sequence that is used to amplify the target gene.
  3.    An oligonucleotide of known sequence that is annealed to the target gene, allowing Taq polymerase to bind.
  4.    A section of DNA on the target gene that is the binding site for Taq polymerase.
 Discuss Question
Answer is Option C. -> An oligonucleotide of known sequence that is annealed to the target gene, allowing Taq polymerase to bind.
Question 6. What type of bonds hold the two strands of DNA together?
  1.    Hydrogen bonds
  2.    Covalent bonds
  3.    Dipole-Dipole bonds
  4.    Ionic bonds
 Discuss Question
Answer is Option A. -> Hydrogen bonds
Question 7. What is the function of DNA Polymerase?
  1.    attach primers to target sequence
  2.    separate the DNA
  3.    attach nucleotides to the target sequence
  4.    separate the fragments according to size
 Discuss Question
Answer is Option C. -> attach nucleotides to the target sequence
Question 8. Which of the following is true regarding PCR
  1.    Denaturation involves heating at 90-98 degree C
  2.    Annealing involves binding of primer between 40-60 degree C
  3.    Primer extension occurs at 72 degree C
  4.    All of these
 Discuss Question
Answer is Option D. -> All of these
Question 9. Why does PCR require a specific type of polymerase?
  1.    Taq polymerase can withstand the range of temperatures in PCR without denaturing.
  2.    Taq polymerase is the most inexpensive type of polymerase.
  3.    Taq polymerase is the most common type of polymerase.
  4.    A specific type of polymerase is unnecessary.
 Discuss Question
Answer is Option A. -> Taq polymerase can withstand the range of temperatures in PCR without denaturing.
Question 10. At what temperature does the Denature step of PCR occur?
  1.    94-96° C
  2.    50-65° C
  3.    72° C
  4.    Any temperature
 Discuss Question
Answer is Option A. -> 94-96° C