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MCQs

Total Questions : 31 | Page 2 of 4 pages
Question 11. In normal phase HPLC, there is a
  1.    polar solvent/non-polar column
  2.    non polar solvent/polar column
  3.    non polar solvent/non-polar column
  4.    any of the above
 Discuss Question
Answer: Option B. -> non polar solvent/polar column
Question 12. Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)?
  1.    Sample recovery
  2.    Ideal for ionic species and large molecules
  3.    All of these
  4.    Greater sensitivity and reusable columns
 Discuss Question
Answer: Option C. -> All of these
Question 13. Which can be used as a mobile phase in HPLC applications?
  1.    Any compound with non-solubility in liquid
  2.    Any of the above
  3.    Any compound with solubility in liquid
  4.    Any compound with limited solubility in liquid
 Discuss Question
Answer: Option C. -> Any compound with solubility in liquid
Question 14. Dwell volume is defined as
  1.    the time required for the gradient to reach the column
  2.    none of these
  3.    the volume of solvent contained in a liquid chromatographic column
  4.    the volume of the column between the point at which solvents are mixed and the beginning of the column
 Discuss Question
Answer: Option D. -> the volume of the column between the point at which solvents are mixed and the beginning of the column
Question 15. Void volume refers to the
  1.    the volume of solvent contained in a liquid chromatographic column
  2.    the volume of the column between the point at which solvents are mixed and the beginning of the column
  3.    total volume of eluent in the column the remainder being taken up by the packing material
  4.    the time required for the gradient to reach the column
 Discuss Question
Answer: Option C. -> total volume of eluent in the column the remainder being taken up by the packing material
Question 16.  HPLC stands for
  1.    High Pressure Liquid Chromatography
  2.    High Performance Liquid Chromatography
  3.    both (a) and (b)
  4.    Highly Placed Liquid Chromatography
 Discuss Question
Answer: Option C. -> both (a) and (b)
Question 17.  The eluent strength is a measure of
  1.    solvent adsorption energy
  2.    solvent absorption energy
  3.    solvent diffusivity
  4.    solvent mixing index
 Discuss Question
Answer: Option A. -> solvent adsorption energy
Question 18.  HPLC methods include
  1.    liquid/liquid (partition) chromatography
  2.    liquid/solid (adsorption) chromatography
  3.    ion exchange and size exclusion chromatography
  4.    all of the above
 Discuss Question
Answer: Option D. -> all of the above
Question 19.  Column efficiency is measured in terms of number of plates which is
  1.    inversely related to the square of the peak width
  2.    directly related to the square of the peak width
  3.    inversely related to the cube root of the peak width
  4.    directly related to the square of the peak width
 Discuss Question
Answer: Option A. -> inversely related to the square of the peak width
Question 20.  For a typical adsorbent such as silica gel, the most popular pore diameters are
  1.    10 and 50 A°
  2.    60 and 100 A°
  3.    100 and 150 A°
  4.    150 and 200 A°
 Discuss Question
Answer: Option B. -> 60 and 100 A°

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