Questions and answers for exam Preparation

MCQs

Total Questions : 120 | Page 4 of 12 pages

Question 31. Which of the following is untrue regarding the classic yeast two-hybrid method?

It is used for the detection of Protein interactions
Method that relies on the interaction of “bait” and “prey” proteins in molecular constructs in yeast
DNA-binding domain and a trans-activation domain don’t necessarily interact
In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein–protein interactions

Discuss Question

Answer: Option C. -> DNA-binding domain and a trans-activation domain don’t necessarily interact
Answer: (c).DNA-binding domain and a trans-activation domain don’t necessarily interact

Question 32. Which of the following is untrue regarding the classic yeast two-hybrid method?

Protein–protein interaction networks of yeast and a small number of other species have been subsequently determined using this method
This technique is a high throughput approach
Each bait and prey construct has to be prepared individually to map interactions between all proteins
It has been systematically applied to study interactions at the whole proteome level

Discuss Question

Answer: Option B. -> This technique is a high throughput approach
Answer: (b).This technique is a high throughput approach

Question 33. If the bait and prey proteins _______ they bring the DNA-binding and trans-activation domains in such close proximity that they reconstitute the function of the transcription activator, turning ____ the expression of a reporter gene as a result. Which of the following is not the correct pair of blanks?

physically interact, on
do not interact, on
do not interact, off
stop interacting, off

Discuss Question

Answer: Option B. -> do not interact, on
Answer: (b).do not interact, on

Question 34. Which of the following is untrue regarding the Predicting Interactions Based on Domain Fusion?

It is based on gene fusion events
Predicting protein–protein interactions is called the “Rosetta stone” method
A fused protein often reveals relationships between its domain components
A fused protein doesn’t necessarily reveal about the relationships between its domain components

Discuss Question

Answer: Option D. -> A fused protein doesn’t necessarily reveal about the relationships between its domain components
Answer: (d).A fused protein doesn’t necessarily reveal about the relationships between its domain components

Question 35. An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 36. Conserved functional sites can be identified by profile and hidden Markov model–based motif and domain search tools such as SMART and InterPro.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 37. Which of the following is incorrect regarding genome economy?

It is a phenomenon of synthesizing more proteins from fewer genes
This is a major strategy that eukaryotic organisms use to achieve a myriad of genotypic diversities only
This is a major strategy that eukaryotic organisms use to achieve a myriad of phenotypic diversities
There are numerous underlying genetic mechanisms to help account for genome economy

Discuss Question

Answer: Option B. -> This is a major strategy that eukaryotic organisms use to achieve a myriad of genotypic diversities only
Answer: (b).This is a major strategy that eukaryotic organisms use to achieve a myriad of genotypic diversities only

Question 38. The remote homology detection helps to shed light on the possible functions of the proteins that previously have no functional information at all.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 39. Which of the following is incorrect regarding GeneQuiz?

It is a web server for protein DNA annotation
It is a web server for protein sequence annotation
It compares a query sequence against databases using BLAST and FASTA to identify homologs with high similarities
It performs domain analysis using the PROSITE and Blocks databases

Discuss Question

Answer: Option A. -> It is a web server for protein DNA annotation
Answer: (a).It is a web server for protein DNA annotation

Question 40. In some circumstances, one mRNA transcript can lead to the translation of more than one protein.