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Total Questions : 91 | Page 5 of 10 pages
Question 41. The chromatography technique in which the stationary phase may be solid or liquid is referred to as ________________
  1.    ion-exchange chromatography
  2.    thin layer chromatography (TLC)
  3.    high performance liquid chromatography (HPLC)
  4.    fast protein liquid chromatography (FPLC)
 Discuss Question
Answer: Option D. -> fast protein liquid chromatography (FPLC)
Answer: (d).fast protein liquid chromatography (FPLC)
Question 42. In FPLC, the sample separates into various zones of sample components and is referred to as ________
  1.    retention time
  2.    peaks
  3.    bands
  4.    pKₐ
 Discuss Question
Answer: Option C. -> bands
Answer: (c).bands
Question 43. Which procedure is represented in the following diagram?Which Procedure Is Represented In The Following Diagram?
  1.    Ammonium sulphate fractionation
  2.    Ultrasonication
  3.    Ammonium sulphate removal by dialysis
  4.    Homogenization
 Discuss Question
Answer: Option C. -> Ammonium sulphate removal by dialysis
Answer: (c).Ammonium sulphate removal by dialysis
Question 44. What does Z represent in the following diagram?What Does Z Represent In The Following Diagram?
  1.    Parafilm
  2.    Glass rod
  3.    Protein solution
  4.    Ethanol-dry ice mixture
 Discuss Question
Answer: Option C. -> Protein solution
Answer: (c).Protein solution
Question 45. In the below diagram of organic solvent fractionation, what does Y represent?In The Below Diagram Of Organic Solvent Fractionation, What ...
  1.    Stirring motor
  2.    Thermometer
  3.    Protein solution
  4.    Dry-ice-ethanol water mixture
 Discuss Question
Answer: Option D. -> Dry-ice-ethanol water mixture
Answer: (d).Dry-ice-ethanol water mixture
Question 46. Which characteristic of the enzyme can be used for purifying it by using FPLC?
  1.    Molecular weight
  2.    Enzyme-substrate
  3.    Electric charge
  4.    Hydrophobicity
 Discuss Question
Answer: Option B. -> Enzyme-substrate
Answer: (b).Enzyme-substrate
Question 47. A preferable technique to perform fractionation is to cool the enzyme solution.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True
Question 48. Which of these is not a basic amino-acid?
  1.    Arginine
  2.    Histidine
  3.    Aspartate
  4.    Lysine
 Discuss Question
Answer: Option C. -> Aspartate
Answer: (c).Aspartate
Question 49. What is the next step after organic solvent fractionation with dry ice – ethanol – water mixture?
  1.    Refrigerated centrifugation at 3000 g for 15 mins
  2.    Drying in freezer
  3.    Supernatant fractionation with ethanol
  4.    Precipitated protein dissolved in 0.01 to 0.05 M cold buffer
 Discuss Question
Answer: Option A. -> Refrigerated centrifugation at 3000 g for 15 mins
Answer: (a).Refrigerated centrifugation at 3000 g for 15 mins
Question 50. Ethanol can be removed from protein precipitate by __________
  1.    lyophilization
  2.    ultrasonication
  3.    homogenization
  4.    dialysis
 Discuss Question
Answer: Option A. -> lyophilization
Answer: (a).lyophilization

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