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MCQs

Total Questions : 91 | Page 3 of 10 pages
Question 21. When pH of enzyme solution is below their isoelectric point, the enzymes will be negatively charged and bind to anion exchangers.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option B. -> False
Answer: (b).False
Question 22. Cation exchange chromatography retains positively charged cations.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True
Question 23. Which of these is not an anion exchanger?
  1.    Diethylaminoethyl (DEAE)
  2.    Quaternary aminoethyl (QAE)
  3.    Sulphopropyl (SP)
  4.    Quaternary ammonium (Q)
 Discuss Question
Answer: Option C. -> Sulphopropyl (SP)
Answer: (c).Sulphopropyl (SP)
Question 24. Peroxidases can be purified using ___________
  1.    gel filtration
  2.    cation exchanger
  3.    anion exchanger
  4.    affinity chromatography
 Discuss Question
Answer: Option C. -> anion exchanger
Answer: (c).anion exchanger
Question 25. Pick the odd one out.
  1.    Carboxymethyl (CM)
  2.    Diethylaminoethyl (DEAE)
  3.    Sulphopropyl (SP)
  4.    Methyl sulphonate (S)
 Discuss Question
Answer: Option B. -> Diethylaminoethyl (DEAE)
Answer: (b).Diethylaminoethyl (DEAE)
Question 26. Alkaline protease is purified by using ________
  1.    DEAE-cellulose
  2.    Q-sepharose
  3.    Amberlite CG-50
  4.    Sephacryl S-300
 Discuss Question
Answer: Option A. -> DEAE-cellulose
Answer: (a).DEAE-cellulose
Question 27. Acetylcholine esterase can be purified by using affinity chromatography with the use of _______ as a ligand.
  1.    ATP
  2.    NADP
  3.    Acrdinium
  4.    Con A
 Discuss Question
Answer: Option C. -> Acrdinium
Answer: (c).Acrdinium
Question 28. The time at which specific analyte elute out is termed as _________
  1.    Kₘ
  2.    half-life
  3.    retention time
  4.    pKₐ
 Discuss Question
Answer: Option C. -> retention time
Answer: (c).retention time
Question 29. _______________ refers to process of isolation and purification of compounds.
  1.    FPLC
  2.    Analytical HPLC
  3.    Preparative HPLC
  4.    TLC
 Discuss Question
Answer: Option C. -> Preparative HPLC
Answer: (c).Preparative HPLC
Question 30. The chromatography in which the sample is forced through a column of the stationary phase by pumping a liquid (mobile phase) at high pressure through the column is referred to as ___________
  1.    affinity chromatography
  2.    fast protein liquid chromatography (FPLC)
  3.    thin layer chromatography (TLC)
  4.    high performance liquid chromatography (HPLC)
 Discuss Question
Answer: Option D. -> high performance liquid chromatography (HPLC)
Answer: (d).high performance liquid chromatography (HPLC)

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