Questions and answers for exam Preparation

MCQs

Total Questions : 165 | Page 6 of 17 pages

Question 51. Which of the given statements is incorrect about Microarray (or microchip) analysis?

It is a new technology in which all of the genes of an organism are represented by oligonucleotide sequences spread out in an 80 x 80 array on microscope slides
The oligonucleotide sequences cannot be synthesized directly on the slide
The oligonucleotides are collectively hybridized to a labeled cDNA library prepared by reverse-transcribing mRNA from cells
The amount of label binding to each oligonucleotide spot reflects the amount of mRNA in the cell

Discuss Question

Answer: Option B. -> The oligonucleotide sequences cannot be synthesized directly on the slide
Answer: (b).The oligonucleotide sequences cannot be synthesized directly on the slide

Question 52. Which of the given statements is incorrect about global gene regulation?

One way to obtain useful information about a genome is to determine which genes are induced or repressed in response to a phase of the cell cycle
Sets of a gene whose expression rises and falls under the same condition are likely to have a related function
Sets of a gene whose expression rises and falls under the same condition are likely to have dissimilar functions
Cell cycle is a developmental phase, or a response to the environment

Discuss Question

Answer: Option C. -> Sets of a gene whose expression rises and falls under the same condition are likely to have dissimilar functions
Answer: (c).Sets of a gene whose expression rises and falls under the same condition are likely to have dissimilar functions

Question 53. In cluster analysis of microarray data– If Xi is the log odds value for gene X at time i, then for two genes X and Y and N observations, a similarity score is calculated. S(X,Y) is also known as the Pearson correlation coefficent. Xoffset and Yoffset can be the mean of the observations on X or Y, respectively, in which case is the standard deviation, or else Xoffset and Yoffset can be set to zero when a reference state is used. Which of the following best represents it?

S(X,Y) = \(\frac{1}{N-2}\) ∑i=1,N . (Xi – Xoffset) (Yi + Yoffset)/ϕxQY
S(X,Y) = \(\frac{1}{N}\) ∑i=1,N . (Xi – Xoffset) (Yi – Yoffset)/ϕxQY
S(X,Y) = \(\frac{1}{N-1}\) ∑i=1,N . (Xi + Xoffset) (Yi + Yoffset)/ϕxQY
S(X,Y) = \(\frac{1}{N}\) ∑i=1,N+2 . (Xi + Xoffset) (Yi – Yoffset)/ϕxQY

Discuss Question

Answer: Option B. -> S(X,Y) = \(\frac{1}{N}\) ∑i=1,N . (Xi – Xoffset) (Yi – Yoffset)/ϕxQY
Answer: (b).S(X,Y) = \(\frac{1}{N}\) ∑i=1,N . (Xi – Xoffset) (Yi – Yoffset)/ϕxQY

Question 54. Once a set of genes that are co-regulated has been found, the promoter regions of these genes may be analyzed for conserved patterns that represent sites of interaction with specific transcription factors.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 55. Which of the given statements is incorrect about Microarray Analysis?

It is designed to detect global changes in transcription in a genome
It provides information about the levels of protein products of the genes
The proteins are first separated in a column on the basis of size and then across a second dimension on a slab on the basis of charge
Labeled protein samples may also be extracted from treated cells and separated by two-dimensional gel electrophoresis

Discuss Question

Answer: Option B. -> It provides information about the levels of protein products of the genes
Answer: (b).It provides information about the levels of protein products of the genes

Question 56. In case of functional genomics– Two general types of approaches are used—one in which a genetic construct is made that interferes with the expression of a particular gene (and sometimes a set of related genes) and a second in which a large number of random mutations are generated in a population of organisms.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 57. In Reverse-genetics analysis of gene function– Even though a particular gene may be _____ ortholog of a gene of known function in another organism, that gene may be acquired by a _____ function.

a highly predicted, similar
a highly predicted, same
a highly predicted, novel
less predicted, novel

Discuss Question

Answer: Option C. -> a highly predicted, novel
Answer: (c).a highly predicted, novel

Question 58. A genome database may also be interfaced with other types of data, such as clinical data.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 59. In addition to the care needed in organizing genome databases, a great deal of human input is needed to annotate the genome manually with information.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 60. The ultimate step in genome analysis is to collect the information found on gene and protein sequences, alignments, gene function and location, protein families and domains, relationships of genes to those in other organisms, chromosomal rearrangements, and so on, into a comprehensive database.