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Total Questions : 136 | Page 5 of 14 pages
Question 41. Which of the following detectors can be used for detection of amino acids in protein hydrolyzates by introducing the reagent dansylchloride in the sample?
  1.    Electrochemical detectors
  2.    Fluorescence detectors
  3.    Refractive index detectors
  4.    Thermal conductivity detectors
 Discuss Question
Answer: Option B. -> Fluorescence detectors
Answer: (b).Fluorescence detectors
Question 42. Which of the following detectors can detect almost any component?
  1.    Combining two UV detectors
  2.    Combining RI and UV detectors
  3.    Mass detector
  4.    Laser-based detectors
 Discuss Question
Answer: Option B. -> Combining RI and UV detectors
Answer: (b).Combining RI and UV detectors
Question 43. Which of the following colour reagents are used in Resin column chromatography?
  1.    Marquis reagent
  2.    Benedict reagent
  3.    Ninhydrin
  4.    Nessler’s reagent
 Discuss Question
Answer: Option D. -> Nessler’s reagent
Answer: (d).Nessler’s reagent
Question 44. Which one of the following methods is the most suitable for amino-acid analysis?
  1.    Gas chromatography
  2.    Ion exchange chromatography
  3.    Paper electrophoresis
  4.    Resin column chromatography
 Discuss Question
Answer: Option D. -> Resin column chromatography
Answer: (d).Resin column chromatography
Question 45. Which of the following amino-acids is measured at a wavelength of 440nm using photometric systems?
  1.    Proline
  2.    Alanine
  3.    Glutamine
  4.    Valine
 Discuss Question
Answer: Option A. -> Proline
Answer: (a).Proline
Question 46. What is the drawback that occurs in using ion exchange chromatography on sulphonated polystyrene resin and colourimetry for amino-acid analysis?
  1.    Less accuracy
  2.    Low resolution
  3.    Inconvenient to handle many individual samples
  4.    Slow in operation
 Discuss Question
Answer: Option C. -> Inconvenient to handle many individual samples
Answer: (c).Inconvenient to handle many individual samples
Question 47. In Automatic amino-acid analyzer, the sample containing ___________ of each amino compound is introduced at the top of the ion exchange column.
  1.    1 to 10 µmoles
  2.    1 to 10 moles
  3.    0.05 to 2 moles
  4.    0.05 to 2 µmoles
 Discuss Question
Answer: Option D. -> 0.05 to 2 µmoles
Answer: (d).0.05 to 2 µmoles
Question 48. Which of the following cannot be analysed using resin column chromatography?
  1.    Peptides
  2.    Amines
  3.    Amino compounds
  4.    Components which are ninhydrin negative
 Discuss Question
Answer: Option D. -> Components which are ninhydrin negative
Answer: (d).Components which are ninhydrin negative
Question 49. Trapped air in pumps of Automatic amino-acid analyzer is eliminated using which of the following ways?
  1.    Suction pump
  2.    De-aerator
  3.    Overcome naturally by pump pressure
  4.    Using pulse damper
 Discuss Question
Answer: Option B. -> De-aerator
Answer: (b).De-aerator
Question 50. What is the drawback that occurs in using gas chromatography for amino-acid analysis?
  1.    Costly
  2.    Cannot be used for proteins or polypeptides
  3.    Slow process
  4.    Complex in operation
 Discuss Question
Answer: Option B. -> Cannot be used for proteins or polypeptides
Answer: (b).Cannot be used for proteins or polypeptides

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