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Total Questions : 76 | Page 2 of 8 pages
Question 11. Which of the following technology is used for whole-blood monitoring in diabetes control?
  1.    Amperometric biosensor
  2.    Potentiometric biosensor
  3.    Optical biosensor
  4.    Calorimetric biosensor
 Discuss Question
Answer: Option C. -> Optical biosensor
Answer: (c).Optical biosensor
Question 12. What does ‘X’ represent in the reaction?
Chromogen (2H) + H₂O₂ \(\underset{X}{\rightarrow}\) Dye + 2H₂O
  1.    Urease
  2.    Luciferase
  3.    Peroxidase
  4.    Penicillinase
 Discuss Question
Answer: Option C. -> Peroxidase
Answer: (c).Peroxidase
Question 13. _______________ is used to detect the presence of bacteria in food or clinical samples.
  1.    Proteolytic enzymes
  2.    Luciferase
  3.    Rennet
  4.    Glucoamylase
 Discuss Question
Answer: Option B. -> Luciferase
Answer: (b).Luciferase
Question 14. What does ‘Y’ represent in the following reaction?
ATP + Y + O₂ \(\underset{Luciferase}{\rightarrow}\) Oxyluciferin + AMP + pyrophosphate + CO₂ + light
  1.    Esters
  2.    Hydrogen peroxide
  3.    D – luciferin
  4.    Uric acid
 Discuss Question
Answer: Option C. -> D – luciferin
Answer: (c).D – luciferin
Question 15. Luciferase is obtained from ________
  1.    firefly
  2.    yeast
  3.    bacillus acidopullulyticus
  4.    malted barley
 Discuss Question
Answer: Option A. -> firefly
Answer: (a).firefly
Question 16. Which of the following statements is not true for biosensors?
  1.    Biosensors convert a biological signal into an electrical signal
  2.    Biosensors are used to determine the concentration of substances and other parameters of biological interest even where they do not utilize a biological system directly
  3.    Biosensors utilize the different biological systems such as enzymes, whole cell metabolism, ligand binding and the antibody-antigen reaction
  4.    Biosensor consists of a vessel, or series of vessels, used to perform a desired conversion by enzymic means
 Discuss Question
Answer: Option D. -> Biosensor consists of a vessel, or series of vessels, used to perform a desired conversion by enzymic means
Answer: (d).Biosensor consists of a vessel, or series of vessels, used to perform a desired conversion by enzymic means
Question 17. If the physical change accompanying the reaction is heat output, the biosensors are referred to as _________________
  1.    potentiometric biosensors
  2.    optical biosensors
  3.    calorimetric biosensors
  4.    amperometric biosensors
 Discuss Question
Answer: Option C. -> calorimetric biosensors
Answer: (c).calorimetric biosensors
Question 18. Which of the following features is not possessed by biosensor?
  1.    The biocatalyst used in the biosensor must be highly specific for the purpose of the analyses
  2.    The reaction occurring in the biosensor should be as independent of such physical parameters
  3.    Active site is mainly constituted by non-polar amino acids for catalysis to take place
  4.    The response from the biosensors should be accurate, precise, reproducible and linear
 Discuss Question
Answer: Option C. -> Active site is mainly constituted by non-polar amino acids for catalysis to take place
Answer: (c).Active site is mainly constituted by non-polar amino acids for catalysis to take place
Question 19. Immobilized enzymes are more preferred over free enzymes in producing biosensors.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True
Question 20. Which of the following property is not possessed by biosensors?
  1.    The probe used in the biosensor must be tiny and biocompatible, having no toxic or antigenic effects
  2.    The biosensor should be cheap, small, portable and capable of being used by semi-skilled operators
  3.    There should be a market for the biosensor
  4.    The specificity of substrate binding is mediated by spatial arrangement of atoms in an enzyme’s substrate binding site as well as substrate.
 Discuss Question
Answer: Option D. -> The specificity of substrate binding is mediated by spatial arrangement of atoms in an enzyme’s substrate binding site as well as substrate.
Answer: (d).The specificity of substrate binding is mediated by spatial arrangement of atoms in an enzyme’s substrate binding site as well as substrate.

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