Why must annealing of primers take place at between 50

Question

Why must annealing of primers take place at between 50 - 65oC?

Options:

A . To prevent Taq polymerase from denaturing, allowing it to catalyse the binding of primers to the target gene.

B . To ensure that primer annealing happens quickly - higher rate of reaction as temperature is high.

C . To allow the formation of hydrogen bonds between the primer and target gene.

D . To prevent the formation of hydrogen bonds between the primer and target gene.

Answer: Option C

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