The efficiency of amplification in PCR in later cycles is reduced due to Options: A . &nbspinsufficient enzyme and time to synthesize mass quantity of DNA B . &nbspreduction in substrate concentration C . &nbspbuild up of PCR product which competes with primers for hybrid formation D . &nbspall of these

The efficiency of amplification in PCR in later cycles is reduced due to

Question

The efficiency of amplification in PCR in later cycles is reduced due to

Options:

A . insufficient enzyme and time to synthesize mass quantity of DNA

B . reduction in substrate concentration

C . build up of PCR product which competes with primers for hybrid formation

D . all of these

Answer: Option D

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