Question
Some of the steps involved in the production of human insulin are given below. Choose the correct sequence
i. Obtaining of gene for human insulin using c-DNA technology
ii. Culturing recombinant E-coli in bioreactors
iii. Purification of human insulin
iv. Insertion of human insulin gene into plasmid
v. Introduction of recombinant plasmid into E.coli
vi. Extraction of recombinant gene product from E-coli
i. Obtaining of gene for human insulin using c-DNA technology
ii. Culturing recombinant E-coli in bioreactors
iii. Purification of human insulin
iv. Insertion of human insulin gene into plasmid
v. Introduction of recombinant plasmid into E.coli
vi. Extraction of recombinant gene product from E-coli
Answer: Option C
:
C
First the human insulin gene is obtained using c-DNA technology and then inserted into a plasmid. The recmbinant plasmid is introduced to E.coli by transformation.The transformed E.coli is selectively cultured inbioreactors and the Insulin protein in extracted and subsequently purified in what is known as downstream processing.
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:
C
First the human insulin gene is obtained using c-DNA technology and then inserted into a plasmid. The recmbinant plasmid is introduced to E.coli by transformation.The transformed E.coli is selectively cultured inbioreactors and the Insulin protein in extracted and subsequently purified in what is known as downstream processing.
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