Question: Select the correct match from the following.
A.Phytoplankton – Protozoa
B.Holozoic nutrition – Fungi
C.Parasitic protists – Slime moulds
D.Symbiosis – Lichens
Answer: Option D
: D

Symbiosis - association between two organisms where both get benefited. Lichens are symbioticassociation between algae and fungi.

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Question 1. In Gram staining of bacteria, the primary stain used is
  1.    Safranin
  2.    Aceto-orcein
  3.    Crystal violet
  4.    iodine solution
Answer: Option C
: C

Primary stain used is crystal violet.Crystal violet stains all thecells blue/purple.
Question 2. Which statement is incorrect?
  1.    Plant virus contains RNA
  2.    Animal virus contains DNA
  3.    T4 contains dsDNA
  4.    TMV contains dsRNA
Answer: Option D
: D

The plant viruses –Tobamo viruses(tobacco mosaic virus) tomato mosaic virus;Potex viruses (potato virus X, papaya mosaic virus); yellow mosaic virus, tobacco necrosis virus, alpha mosaic virus; satellite tobacco necrosis virus and some animal viruses –Togaviurs,Picornaviruses (Polivirus), etc., contain single stranded RNA.
Question 3.  A bacterium divides every 35 minutes. If a culture containing 105  cells per mL is grown for 175 min, what will be the cell concentration per mL after 175 minutes?
  1.      175 x 105 cells
  2.     32 X 105 cells
  3.     5 x 105 cells
  4.    35 x 105 cells
Answer: Option B
: B

The time taken by a bacterium to divide is calledgeneration time (G) or doubling time,since each parent cell divides into two daughter cells. In this particular culture, the doubling time is 35 minutes. We are asked what will be cell concentration after 175 min. Given,thegeneration time Gis 35 min.The total time elapsed (t)=175 min. Then the cells would have divided or doubled 5 times (175/35=5), in this period. This means n=5 (wheren is the number of generations). After one doubling, a single cell would become 2 cells. In the second generation, these 2 cells would double to 4 cells and so on. This gives a simple formula= 2n. Given that n=5, then 25=32. This calculation, assumes that the starting culture contained a single cell. In the problem, we are informed that the culture contained an initial cell concentration of 105cells/mL. Therefore, the final cell concentration after 175 min should be 105X 32 cells/mL.

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